Analytical Service Requisition Form
Sample and primer information for DNA sequencing
- Please provide purified PCR product for DNA sequencing. The accepted purified PCR product purity (260nm/280nm value of spectrophotometer) should be 1.7-1.9.
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Kindly mention the product size and concentration of the samples. Recommended product size and concentrationshould be as follows
Product size (base pair) Concentration (ng/µL) PCR product: 100-200 bp 10-20 ng 200-500 bp 20-30 ng 500-1000 bp 30-50 ng >1000 bp >50 ng - Kindly provide a gel picture of purified PCR product (please load 1 or 2 µL of each sample in gel).
- Please give 10 µL volume of purified PCR product for per reaction.
- Please mention the annealing temperature and volume of primer was used during PCR set up and also the final volume of PCR.
- Please provide at least four reactions per primer.
- Please provide 10µLworking primer for per reaction.
- Please provide a blank C.D with your sample to receive your result.