Welcome To Genetic Engineering and Biotechnology Research Laboratory
Overview:
The Genetic Engineering and Biotechnology Research Laboratory (GEBR) is one of the 13 research discipline of Centre for Advanced Research in Sciences (CARS) & was launched in January, 2006 at (CARS), University Dhaka. The laboratory is well equipped for various research in molecular biology, human genetics, forensic & biotechnology. All instruments are in operation at the "state-of-the-art” condition. This laboratory has been founded for cutting edge research, education and training to support students of higher degree, public, governmental and non-governmental organization. This laboratory practices international SOP guided techniques to achieve standard quality data.
Research Area:
Research Interest:
¨ Population Genetics
¨ Genetic diseases
¨ Type 2 Diabetes
¨ Forensic Genetics
¨ Bioinformatics
Services:
Services:
· DNA Sequencing (only from purified PCR product)
· DNA profiling
·PCR (Instrument use only)
· DNA/RNA extraction (Space and instrument only)
· DNA quantitation by Gel electrophoresis
· DNA quantitation by Nanodrop spectrophotometer
· DNA Profiling (non-case)
Facilities:
The Genetic Engineering and Biotechnology Research laboratory (GEBRL) of (CARS) Dhaka University is well designed and has most of the modern equipment needed to carry out research on population genetics, genetic disease susceptibility, epigenetics, including method development and technology transfer for Forensic Genetics.
Ø SeqStudioTM Genetic Analyzer
Ø ProFlex Thermal Cycler (PCR Machine)
Ø Electrophoresis system
Ø Gel Documentation system
Ø High speed refrigerated centrifuge
Ø PCR cabinet
Ø ELISA
Ø -20oC and -40oC freezer
Ø Other necessary instruments
Personnel:
Personnel:
Chief Scientist: Gazi Nurun Nahar Sultana, Ph.D.
Principal Scientist: Rokeya Begum, Ph.D.
Postdoctoral Fellow: Dr. Israt Jahan
Technical support
Sharif Moshiur Rahman, Senior Technical staff
Current Projects:
| Sl | Title | Year |
|---|---|---|
| 1 | Investigation of the Association of Mitochondrial NADH Dehydrogenase1, 2 And 3 Mutations with Type 2 Diabetes Mellitus: Grant received from Dhaka University | 2021-2022 |
| 2 | Association of Mitochondrial Cytochrome b (MT-CYB) Mutations with Type 2 Diabetes Mellitus: Grant received from Dhaka University | 2022-2023 |
| 3 | Peopling of Bangladesh”: Grant received from National Geography Project in collaboration with | 2018-2020 |
| 4 | Haplotyping of COVID-19 patients using mtDNA marker: Grant received from Dhaka University | 2021-2022 |
| 5 | Identification of foreign meat by genetic marker”: Grant received from Dhaka University | 2022-2023 |
| 6 | Altered DNA methylation in Breast Cancer Patients”: Grant received from Ministry of Health in collaboration with NICRH, GOB | 2022-2024 |
Collaborations:
| Sl | Organization | Description |
|---|---|---|
| 1 | Department of Microbiology, Jagannath University | Jointly supervised MS thesis work |
| 2 | Department of Biochemistry and Molecular Biology, Dhaka University of Dhaka | Jointly supervised MS thesis work |
| 3 | Department of Genetic Engineering and Biotechnology, Dhaka University of Dhaka | Jointly supervised MS thesis work |
| 4 | Bangladesh Institute of Research and Rehabilitation in Diabetes, Endocrine and Metabolic Disorders (BIRDEM), | Conducting a joint research on "Association of genetic and mitochondrial DNA mutations with Type 2 Diabetes Mellitus (T2DM) in people of Bangladesh” |
| 5 | Department of Antomy, Bangabandhu Sheikh Mujib Medical University (BSMMU) | Conducting a joint research on "Hypertropic Cardiomyopathy in people of Bangladesh" |
| 6 | Department of zoology, Banaras Hindu University (BHU), Varanasi, India | Both thee laboratories jointly working on "Peopling of Bangladesh" funded by National Geography Project. |
| 7 | Enstonia Biocentre, Tartu, Estonia | GEBRL has received a grant from Estonia Biocentre to study on the "Migration History of Rohingya Population" on 2018-2022 (completed) |
| 8 | National Institute of Cancer Research Hospital (NIRCH) | NICRH and GEBRL has been jointly awarded a project grant on " 1. “Altered DNA methylation in Breast Cancer Patients” from the Ministry of Health, GOB, for two years. 2023-2024. |
Publications:
| Sl. | Article | Type | Year | Link |
|---|---|---|---|---|
| 1 | Arindita Das, Nazmul Ahsan, Rokeya Begum, Gazi Nurun Nahar Sultana. Human Gene, Elsevier Publication | Journal | 2023 | Link |
| 2 | Gazi Nurun Nahar Sultana, Arindita Das, Khalida Akhtaar, Chowdhury Faiz Hossain and M Anarul Islam. STUDY OF COVID-19 CASE WITH MULTIPLE COMORBIDITIES: SEVERE TO SURVIVAL Bioresource Communications; 9 (1): 1177-1179 | Journal | 2023 | Link |
| 3 | 1. Israfil, H.M., Begum, R., Sultana, N.N.G. 2023. Validation of Gender Identification by “Alu” Insertion on Y Chromosome for Forensic Mass Disaster. Current Forensic Science, 1, 1-4. | Journal | 2023 | Link |
| 4 | Quantitative Analysis of serum cell free DNA as predictive and prognostic marker in breast cancer patients | Journal | 2023 | Link |
| 5 | Sultana, Gazi Nurun, Dilruba Sharmin, Iffat Jahan, Mohammad Asaduzzaman, Rokeya Begum, Anindita Paul, and M Mizanur Rahman. 2022. “Alu-Deletion in Bangladeshi Women: Scopes of Clinical Utility for the Detection of Early Breast Cancer As a Biomarker”. Asian Pacific Journal of Cancer Biology 7 (1), 43-49. https://doi.org/10.31557/apjcb.2022.7.1.43-49. | Journal | 2022 | Link |
| 6 | GNN Sultana, A Srivastava, K Akhtaar, PP Singh, MA Islam, G Chaubey. Studying C-reactive protein and D-dimer levels in blood may prevent severe complications: A Study in Bangladeshi COVID-19 patients. Genet. Evolutionary and Population Genetics. https://doi.org/10.3389/fgene.2022.96659 | Journal | 2022 | |
| 7 | GNN Sultana, D Sharmin, I Jahan, M Asaduzzaman, R Begum, A Paul. Alu-deletion in Bangladeshi Women: Scopes of Clinical Utility for the Detection of Early Breast Cancer as a Biomarker. Asian Pacific Journal of Cancer Biology, 7 (1): 43-49 | Journal | 2022 | |
| 8 | M Kabir, M Imam, CF Hossain, GNN Sultana. Fluorescence Resonance Energy Transfer Based Biosensor from Thermophilic Bacterial Periplasmic Binding Protein to Measure Branched-chain and Aromatic Amino Acids. Journal of Advances in Biology & Biotechnology, 25 (6): 24-3 | Journal | 2022 | |
| 9 | Begum, R., Sajib, A. A., Islam, A. B. M. K., Sarker, S. K., Islam, M. S., Saha, N., . . Akhteruzzaman, S. (2021). MUT gene variants in patients with methylmalonic acidemia in Bangladeshi population and their distinguishing metabolic profiles. Meta Gene, 30, 100954. doi: https://doi.org/10.1016/j.mgene.2021.100954 | Journal | 2021 | Link |
| 10 | Rahman MM, Sultana GNN, Sarker MAK, Hossain MM, Alam MS, Rahman MA, Ahad A, Islam MM, Begum R. 2021. Alteration of P53, hMLH1, and HER2 Gene in Bangladeshi Gastric Cancer Patients: Their Association with H. pylori Infection and Clinicopathological Factors. Middle East Journal of Cancer 12(3):332-341. doi: 10.30476/mejc.2020.84147.1207. | Journal | 2021 | Link |
| 11 | Prajjval Pratap Singh, Shani Vishwakarma, Gazi Nurun Nahar Sultana, Arno Pilvar, Monika Karmin, Siiri Rootsi, Richard Villems, Mait Metspalu, Doron M Behar, Toomas Kivisild, George van Driem, Gyaneshwer Chaubey. Dissecting the paternal founders of Mundari (Austroasiatic) speakers associated with the language dispersal in South Asia. European journal of human genetics; 29 (3): 528-532 | Journal | 2021 | |
| 12 | Gazi Nurun Nahar Sultana, M. Islam, R Khan, A Islam, H Mahmud, S Raihan. Study on mitochondrial Atpase6 gene polymorphisms as a genetic risk factor for breast cancer in Bangladeshi women. International Journal of Scientific Research in Dental and Medical Sciences; 3 (1): 18022 | Journal | 2021 | |
| 13 | M Mizanur Rahman, Gazi Nurun Nahar Sultana, Mohammed Abu Kawsar Sarker, Md Hossain, Mohd Alam, Md Rahman, Abdul Ahad, Md Islam, Rokeya Begum. Alteration of P53, hMLH1, and HER2 Gene in Bangladeshi Gastric Cancer Patients: Their Association with H. pylori Infection and Clinicopathological Factors. Middle Eastern Journal of cancer; 12 (3): 332-341.of | Journal | 2021 | |
| 14 | Prajjval Pratap Singh, Anshika Srivastava, Gazi Nurun Nahar Sultana, Nargis Khanam, Abhishek Pathak, Prashanth Suravajhala, Royana Singh, Pankaj Shrivastava, George van Driem, Kumarasamy Thangaraj, Gyaneshwer Chaubey. The major genetic risk factor for severe COVID-19 does not show any association among South Asian populations. Scientific Report, 11 (1): 1-4 | Journal | 2021 | |
| 15 | Anshika Srivastava, Audditiya Bandopadhyay, Debashurti Das, Rudra Kumar Pandey, Vanya Singh, Nargis Khanam, Nikhil Srivastava, Prajjval Pratap Singh, Pavan Kumar Dubey, Abhishek Pathak, Pranav Gupta, Niraj Rai, Gazi Nurun Nahar Sultana, Gyaneshwer Chaubey. Genetic association of ACE2 rs2285666 polymorphism with COVID-19 spatial distribution in India. Frontiers in genetics; 11: 1163 | Journal | 2020 | Link |
| 16 | Rahman, M Mizanur, M. Abu Kawsar Sarker, Md Monowar Hossain, Mohd. Sahajadul Alam, Md. Monzurul Islam, Laila Shirin, Rokeya Begum and Gazi Nurun Nahar Sultana. “Association of p53 Gene Mutation With Helicobacter pylori Infection in Gastric Cancer Patients and Its Correlation With Clinicopathological and Environmental Factors.” World journal of oncology (2019). | Journal | 2019 | Link |
| 17 | Sheikh Ariful Hoque, Aksara Thongprachum, Sayaka Takanashi, Rokeya Begum, Ummay Nasrin Sultana, Tania Hossain, Pattara Khamrin, Shoko Okitsu, Satoshi Hayakawa, and Hiroshi Ushijima, 'Alarming Situation of Spreading Enteric Viruses through Sewage Water in Dhaka City: Molecular Epidemiological Evidences', Food and Environmental Virology, 11 (2019), 65-75. | Journal | 2019 | Link |
| 18 | Kai Tätte, Luca Pagani, Ajai K Pathak, Sulev Kõks, Binh Ho Duy, Xuan Dung Ho, Gazi Nurun Nahar Sultana, Mohd Istiaq Sharif, Md Asaduzzaman, Doron M Behar, Yarin Hadid, Richard Villems, Gyaneshwer Chaubey, Toomas Kivisild, Mait Metspalu. The genetic legacy of continental scale admixture in Indian Austroasiatic speakers. Scientific Reports; 8 (3818): 1-9 | Journal | 2019 | Link |
| 19 | Mohammad Sahajadul Alam, M. Mizanur Rahman, Md. Monowar Hossain, Mohammed Abu Kawsar Sarker, Md. Monzurul Islam, Rokeya Begum, Most Umme Habiba, Gazi Nurun Nahar Sultana, Biography: Mutated hMLH1 Gene in Bangladeshi Gastric Cancer Patients: Its Relevance to Clinicopathological Factors, International Journal of Clinical Oncology and Cancer Research. Vol. 3, No. 4 ( 2018), pp. 49-54. doi: 10.11648/j.ijcocr.20180304.11 | Journal | 2018 | Link |
| 20 | MM Rahman, MAK Sarker, MM Hossain, MS Alam, MM Islam, L Shirin, Rokeya Sultana, Gazi Nurun Nahar Sultana. Association of p53 gene mutation with helicobacter pylori infection in gastric cancer patients and its correlation with clinicopathological and environmental factors. World Journal of Oncology; 10 (1), 46 | Journal | 2018 | Link |
| 21 | Begum, R., Choudhury, S., Latif, Z. A., Sultana G. N. N. (2016). Effect of PTPRδ rs17584499 C/T Polymorphism on Therapeutic Efficacy of Metformin in Bangladeshi Patients with Type 2 Diabetes. Biores Comm, 2(1), 182-188 | Journal | 2016 | |
| 22 | Begum, R., Shahid, S., Razzaque, S., Jesmin, & Seraj, Z. I. (2016). Variability in amylose content of Bangladeshi rice cultivars due to unique SNPs in Waxy allele. Journal of Cereal Science, 71, 1-9. doi:http://dx.doi.org/10.1016/j.jcs.2016.07.006 | Journal | 2016 | Link |
| 23 | Sultana GNN, Begum R, Akhter H, Shamim Z, Rahim MA and Chubey G.(2016). The Complete Spectrum of Beta (β) Thalassemia Mutations in Bangladeshi Population. Austin Biomark Diagn, 3(1), 1024 | Journal | 2016 | |
| 24 | Gazi Nurun Nahar Sultana, ASM Ali Ashraf, and Rokeya Begum. (2016) Use of Forensic DNA Technology for Criminal Investigation: Global Trends and Emerging Practices in Bangladesh. In: Intelligence, national security, and foreign policy: a South Asian narrative. Eds.: Asm Ali Ashraf. BILIA, 109-127 | Book | 2016 | |
| 25 | Roy, R. R., Sultana, G. N., Begum, R., Shahinuzzaman, A. D., & Sharif, M. I. (2016). The Aspect of NK2 Transcription Factor Related Locus-5 (NKX2.5) Gene Mutations in Bangladeshi Atrial Septal Defect (ASD) patients and 2D Relationship with their Age. Mymensingh medical journal : MMJ, 25(1), 79-84 | Journal | 2016 | |
| 26 | G Sultana, R Begum, H Akhter, Z Shamim, MA Rahim, G Chubey. The complete Spectrum of beta (β) thalassemia mutations in Bangladeshi population. Austin Biomarker Diagnosis; 3 (1): 1024 | Journal | 2016 | Link |
| 27 | Luca Pagani, Daniel John Lawson, Evelyn Jagoda, Alexander Mörseburg, Anders Eriksson, Mario Mitt, Florian Clemente, Georgi Hudjashov, Michael DeGiorgio, Lauri Saag, Jeffrey D Wall, Alexia Cardona, Reedik Mägi, Melissa A Wilson Sayres, Sarah Kaewert, Charlotte Inchley, Christiana L Scheib, Mari Järve, Monika Karmin, Guy S Jacobs, Tiago Antao, Florin Mircea Iliescu, Alena Kushniarevich, Qasim Ayub, Chris Tyler-Smith, Yali Xue, Bayazit Yunusbayev, Kristiina Tambets, Chandana Basu Mallick, Lehti Saag, Elvira Pocheshkhova, George Andriadze, Craig Muller, Michael C Westaway, David M Lambert, Grigor Zoraqi, Shahlo Turdikulova, Dilbar Dalimova, Zhaxylyk Sabitov, Gazi Nurun Nahar Sultana, et.al. Genomic analyses inform on migration events during the peopling of Eurasia. Nature; 538 (7624): 238-242. | Journal | 2016 | Link |
| 28 | Bari, M. L., Simol, H. A., Khandoker, N., Begum, R., & Sultana, U. N. (2015). Potential Human Health Risks of Tannery Waste-contaminated Poultry Feed. Journal of Health and Pollution, 5(9), 68-77. doi: doi:10.5696/2156-9614-5-9.68 | Journal | 2015 | |
| 29 | Sultana, G. N. N., Begum, R., Asaduzzaman, M., Ahmed, T. and Akhtar, M. (2015). Genetic Analysis of Y-Chromosome17 STR in Four Indigenous Populations from Bandarban. Biores Comm, 1(1), 32-35. | Journal | 2015 | |
| 30 | Begum, R., Elias, S., Rahman, M. S., Ferdouse, A., Shozib, H., Ali, M. M., & Seraj, Z. (2014). Genotypic and phenotypic relatedness of a farmer-discovered variant with high-yielding rice growing in the same field. Plant Systematics and Evolution, 1-15. doi: 10.1007/s00606-014-1085-x | Journal | 2014 | |
| 31 | Sultana, G., Tuli, J., Begum, R. and Tamang, R. (2014) Mitochondrial DNA Control Region Variation from Bangladesh: Sequence Analysis for the Establishment of a Forensic Database. Forensic Medicine and Anatomy Research, 2, 95-100. doi: 10.4236/fmar.2014.24016 | Journal | 2014 | |
| 32 | Sultana GNN, Shahinuzzaman ADA, Begum R, Jahan I and Rahman MM. (2014) Genetic Variations of Mitochondrial Cytochrome B and Breast Cancer. Austin Biomark Diagn, 1(2): 5. | Journal | 2014 | |
| 33 | Gazi Nurun Nahar Sultana, Hasina Akhter Simol, Rokeya Begum, Zakaria Shamim, M. Anwar Hossain (2014). Status of Iron, Magnesium and Zinc in β-Thalassemia Patients of Bangladesh. Online International Interdisciplinary Research Journal, 1V (08), 76-83 | Journal | 2014 | |
| 34 | Gazi Nurun Nahar Sultana, Atiqur Rahman M, Shahinuzzaman ADA, Abdullah ABM, Proyash Roy and Rokeya Begum (2014) Hutchinson-gilford progeria syndrome (hgps) with mitochondrial DNA (mtDNA) hv1 control region mutations. Unique journal of pharmaceutical and biological sciences, 02 (01), 29-32 | Journal | 2014 | |
| 35 | Gazi Nurun Nahar Sultana, Rokeya Begum, Atiqur Rahman, Hosne Ara B. Shahinuzzaman ADA, Jannatul F., Zakaria S., and Anwar H, (2011) Advancement of genetic research in Bangladesh: Illuminating the genetic architecture of life science J. Asia, Soc. Bangladesh, Sci., 37(2), 391-403 | Journal | 2011 | |
| 36 | Gazi Nurun Nahar Sultana, Atiqur Rahman, M. Manjurul Karim, ADA Shahinuzzaman, Rokeya Begum and Rowsan Ara Begum (2011) Breast cancer risk associated mitochondrial NADH-dehydrogenase subunit-3 (ND3) polymorphism. Journal of Medical Genetics and Genomics, 3, 131-135. | Journal | 2011 | |
| 37 | Zeba I.Seraj, Laisa A Lisa, M. Rafiqul Islam, Rokeya Begum and Deepok K. Das (2006) Genetic diversity of saline coastal rice (Oryza sativa L) landraces of Bangladesh. In: Abiotic stress tolerance of plants. Towards the improvement of global environment and food. Eds.: Rai, Aswani K and Takabe, Teruhiro. Springer, 229-244 | Journal | 2006 |